A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:13Introduction
- 01:47Tools and reagents
- 01:56Dissection of olfactory bulb
- 02:38Dissection of frontal cortex
- 03:08Dissection of striatum
- 04:02Dissection of hippocampus
- 05:02Conclusion
Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.
