Tissue Culture
Tissue Culture - Related Videos
09:35
Histotypic Tissue Culture
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2023
Although two-dimensional tissue culture has been common for some time, cells behave more realistically in a three-dimensional culture, and more closely mimics native tissue. This video introduces histotypic tissue culture, where the growth and propagation of one cell line is done in an engineered three-dimensional matrix to reach high cell density. Here, we show the harvesting of cells from donor tissue, followed by cell culture on an engineered construct.
08:45
Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
02:57
Plant Tissue Culture
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2020
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species. Plant tissue culture depends on the ability of plant tissue to give rise to an entire new plant when provided with a growth medium and appropriate environment. This ability of plant cells or tissues is termed ‘totipotency.’ The fundamental steps...
07:45
Explant Culture of Neural Tissue
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2023
The intricate structure of the vertebrate nervous system arises from a complex series of events involving cell differentiation, cell migration, and changes in cell morphology. Studying these processes is essential to our understanding of nervous system function as well as our ability to diagnose and treat disorders that result from abnormal development. However, neural tissues are relatively inaccessible for experimental manipulations, especially in embryonic mammals. As a result, many...
08:47
Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System
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Cited by 10 •
2015
Here, we present an ex vivo flow model in which murine cardiac valves can be cultured allowing the study of the biology of the valve.
FAQs
Sterility and environmental control are central because contamination or unsuitable conditions can prevent cells, tissues, or organs from surviving and dividing. In tissue culture, researchers regulate temperature, pH, nutrient availability, and signaling factors to create a defined setting. These variables determine whether cells remain viable, proliferate, differentiate, or regenerate.
Signaling factors help guide cellular behavior beyond simple survival and division. Depending on the conditions supplied in the culture environment, they can support continued growth, encourage differentiation into specialized cell types, or contribute to tissue regeneration. Their inclusion allows researchers to examine how controlled biological cues influence development and cell function.
A defined environment allows researchers to examine biological processes while controlling important external conditions. By maintaining suitable temperature, pH, nutrients, and signaling factors, investigators can relate observed changes to selected treatments or experimental variables. This supports studies of cell function, development, disease processes, and responses to drugs without the complexity of an entire organism.
The procedure begins with a small tissue sample, which is placed on sterile nutrient media under laboratory conditions. Researchers maintain appropriate temperature, pH, nutrients, and signaling factors so the material can remain viable and develop. Depending on the supplied conditions, the culture may support cell division, differentiation, or regeneration for subsequent biological study.
Researchers can evaluate whether cultured material survives, divides, differentiates, or regenerates under selected conditions. These outcomes provide information about cell function and development, while changes after drug or treatment exposure can reveal biological responses. Because the environment is controlled, tissue culture helps connect specific conditions with measurable cellular or tissue-level effects.
Tissue culture provides a controlled setting for examining disease processes and testing how cells or tissues respond to drugs and other treatments. Researchers can observe biological effects under defined laboratory conditions rather than relying only on complex whole-organism responses. This makes the approach useful for investigating altered cell function and treatment-related outcomes.
Beyond studying development and cell function, tissue culture contributes to plant propagation, regenerative medicine, and production of specialized biological materials. In plant systems, controlled growth can support the generation of new plant material. In regenerative medicine, the ability to maintain and encourage tissue development supports research into restoring or producing biological structures.