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脑浸润白细胞的分离
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Brain-infiltrating Leukocytes
DOI:

06:44 min

June 13, 2011

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Chapters

  • 00:05Title
  • 01:04Intracranial Virus Injection
  • 02:15Brain-infiltrating Leukocyte Cell Preparation
  • 04:41Flow Cytometric Immunophenotyping
  • 05:44Representative Immunophenotype of Brain-infiltrating Leukocytes at 24 hr Post-infection
  • 06:28Conclusion

Summary

Automatic Translation

一个快速的方法来获得从小鼠脑组织浸润的白细胞描述。这种方法利用了连续Percoll梯度和不连续的聚蔗糖梯度选择和净化白细胞富集层。然后可能会被隔离白细胞流式细胞仪测量的特点。

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