JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
脑浸润白细胞的分离
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Isolation of Brain-infiltrating Leukocytes
Please note that all translations are automatically generated.
Click here for the English version.
脑浸润白细胞的分离
DOI:
10.3791/2747-v
•
06:44 min
•
June 13, 2011
•
Reghann G. LaFrance-Corey*
1
,
Charles L. Howe*
1
1
Department of Neurology
,
Mayo Clinic College of Medicine
Chapters
00:05
Title
01:04
Intracranial Virus Injection
02:15
Brain-infiltrating Leukocyte Cell Preparation
04:41
Flow Cytometric Immunophenotyping
05:44
Representative Immunophenotype of Brain-infiltrating Leukocytes at 24 hr Post-infection
06:28
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
一个快速的方法来获得从小鼠脑组织浸润的白细胞描述。这种方法利用了连续Percoll梯度和不连续的聚蔗糖梯度选择和净化白细胞富集层。然后可能会被隔离白细胞流式细胞仪测量的特点。
Tags
Brain-infiltrating Leukocytes
BILs
Mice
Theiler’s Murine Encephalomyelitis Virus
TMEV
Intracranial Injection Technique
Leukocyte Purification
Brain Homogenization
Percoll Gradient
Cell Suspension
Ficoll-Paque Plus Gradient
Immunophenotyping
Flow Cytometry
Innate Immune Cells
Gr1+
CD11b+
F4/80+ Cells
Immune Response
Acute Infection
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
心脏功能免疫细胞的分离
小鼠肺树突状细胞的分离
脑幽静的白细胞的分离与分析<em>伯氏疟原虫</emANKA感染的小鼠
生物能量和氧化突发:协议用于人类白细胞和血小板的隔离与评价
对白细胞从流程的分析招聘基质细胞的影响
从人类母胎界面分离白细胞
从小鼠组织在母胎界面分离白细胞
从小鼠皮肤使用酶消化和梯度分离提取白细胞浸润的
Marginating肺白细胞的选择性收获
Marginating肝白细胞的选择性收获
Read Article