Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Isolation of Brain-infiltrating Leukocytes
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Isolation of Brain-infiltrating Leukocytes
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
Journal (Immunology and Infection)
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
Journal (Immunology and Infection)
In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
 
Click here for the English version

Isolation of Brain-infiltrating Leukocytes

Article DOI: 10.3791/2747-v • 06:44 min • June 13th, 2011
June 13th, 2011 •
*1, *1
1Department of Neurology, Mayo Clinic College of Medicine
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.

Tags

Brain-infiltrating Leukocytes BILs Mice Theiler's Murine Encephalomyelitis Virus TMEV Intracranial Injection Technique Leukocyte Purification Brain Homogenization Percoll Gradient Cell Suspension Ficoll-Paque Plus Gradient Immunophenotyping Flow Cytometry Innate Immune Cells Gr1+ CD11b+ F4/80+ Cells Immune Response Acute Infection
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter