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Neuroscience

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In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy
 

In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

Article DOI: 10.3791/2760
January 5th, 2012

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Summary January 5th, 2012

A minimally invasive protocol to stabilize the mouse spinal column and perform repetitive in vivo spinal cord imaging using two-photon microscopy is described. This method combines a spinal stabilization device and an anesthetic regimen to minimize respiratory-induced movements and produce raw imaging data that require no alignment or other post-processing.

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