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Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
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Biology
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Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)

DOI:
10.3791/2781-v

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10:15 min

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August 06, 2011

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, , , ,
1Centre for Infectious Diseases and Microbiology,University of Sydney

Chapters

  • 00:05Title
  • 01:20Preparation of Membrane
  • 04:01Hybridization and Detection
  • 08:12Representative mPCR/RLB Results
  • 09:56Conclusion

Summary

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An inexpensive, high throughput method for simultaneous detection of up to 43 molecular targets is described. Applications of mPCR/RLB include microbial typing and detection of multiple pathogens from clinical samples.

Tags

Multiplex PCRReverse Line Blot Hybridization AssayMolecular TargetsProbe HybridizationNylon MembraneAmine Modified ProbesBiotin Modified PrimersStreptavidin-peroxidaseChemiluminescent SubstratePhotosensitive FilmSetup CostsConsumable CostsHigh ThroughputShort Turnaround TimeSequence Variation DetectionAntibiotic Resistance GenesMethicillin-resistant Staphylococcus Aureus TypingSalmonella Sp TypingMolecular Serotyping Of Streptococcus Pneumoniae And Streptococcus AgalactiaeEnterovirus IdentificationMycobacterium Sp Detection

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