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Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging

Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging

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11:24 min

June 22, 2011

DOI:

11:24 min
June 22, 2011

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Summary

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A method is described for the preparation of single living photoreceptor cells from different vertebrate species for fluorescence imaging. The method can be used to image the fluorescence of endogenous fluorophores, such as NADH or vitamin A, or that of exogenously added fluorescent dyes sensitive to Ca2+ or other factors.

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