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JoVE Journal
Biology

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Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
 

Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety

Article DOI: 10.3791/2822
July 24th, 2011

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Summary July 24th, 2011

Lentiviruses are a valuable research tool for exploring gene function; however, researchers may wish to avoid production of pantropic lentivirus encoding known or suspected oncogenes. As an alternative, we present a safer protocol for use of ecotropic lentivirus on human cells modified to express the ecotropic receptor mSlc7a1.

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