Journal
/
Medicine
/
Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice
JoVE Journal
Medicine
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

DOI:
10.3791/2879-v

•

13:01 min

•

May 08, 2011

•

, , , ,
1Department of Neurosurgery,University of Florida, 2Department of Anatomical Sciences,Shiraz University of Medical Sciences

Chapters

  • 00:05Title
  • 00:43Introduction
  • 01:57Suture Preparation
  • 02:40Animal Preparation
  • 04:19Koizumi’s Method
  • 06:04Reperfusion
  • 06:27Longa’s Method
  • 09:19Neurological Evaluation After MCAO
  • 09:26Score 1
  • 09:41Score 2
  • 09:50Score 3
  • 10:03Score 4
  • 10:12Staining and Quantitative Measurement of Brain Infarct Volume
  • 10:57Representative Results
  • 11:55Conclusion

Summary

Automatic Translation

Automatic Translation

The intraluminal middle cerebral artery occlusion (MCAO) model is the most frequent used model among experimental ischemic stroke models. Here we will demonstrate the entire model in detail with the guide of Laser Doppler flowmetry, and its representative results.

Tags

Intraluminal Middle Cerebral Artery OcclusionMCAO ModelIschemic StrokeLaser Doppler Flowmetry GuidanceMiceThird Leading Cause Of DeathLeading Cause Of DisabilityCostUnited StatesRatModificationMolecular MechanismsBrain InjuryTherapeutic ModalitiesNoninvasive MethodTransgenic StrainsKnockout StrainsFocal Cerebral IschemiaIntraluminal SutureInternal Carotid ArteryBase Of The MCASpontaneous ReperfusionPermanent RetentionVideo DemonstrationDrawbacksPitfalls235-triphenyltetrazolium Chloride (TTC)Cerebral Infarction

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2879?article_type=v

Modeling Stroke in Mice – Middle Cerebral Artery Occlusion with the Filament Model
check_url/2879?article_type=v

Mouse Model of Middle Cerebral Artery Occlusion
check_url/2879?article_type=v

Bilateral Common Carotid Artery Occlusion as an Adequate Preconditioning Stimulus to Induce Early Ischemic Tolerance to Focal Cerebral Ischemia
check_url/2879?article_type=v

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat
check_url/2879?article_type=v

Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion
check_url/2879?article_type=v

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion
check_url/2879?article_type=v

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery
check_url/2879?article_type=v

Embolic Middle Cerebral Artery Occlusion (MCAO) for Ischemic Stroke with Homologous Blood Clots in Rats
check_url/2879?article_type=v

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice
check_url/2879?article_type=v

Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

Read Article