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Extraction of High Molecular Weight DNA from Microbial Mats
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Extraction of High Molecular Weight DNA from Microbial Mats

Article DOI: 10.3791/2887-v • 09:30 min • July 7th, 2011
July 7th, 2011 •
1, 1, 1
1Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina

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We provide an improved protocol for extracting high molecular weight DNA from hypersaline microbial mats. Microbial cells are separated from the mat matrix prior to DNA extraction and purification. This enhances the concentrations, quality, and size of the DNA. The protocol may be used for other refractory samples.

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High Molecular Weight DNA Microbial Mats Metagenomic Data Analysis DNA Extraction HMW Community DNA Environmental Mat Samples Extracellular Polymeric Substances (EPS) Salts Direct Lysis Harsh Extraction Methods Fragmented DNA Large-insert Vector Cloning Improved Methodology Hypersaline Microbial Mats Blending And Differential Centrifugation Mechanical And Chemical Procedures DNA Purification A260/280 Ratio 16S RRNA Genes Amplification
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