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JoVE Journal
Bioengineering

This content is Open Access.

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
 

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Article DOI: 10.3791/2925
February 9th, 2012

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Summary February 9th, 2012

Fluorescence Lifetime Imaging (FLIM) has emerged as a key technique to image the environment and interaction of specific proteins and dyes in living cells. FLIM of fluorescent molecular rotors allows mapping of viscosity in living cells.

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