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JoVE Journal Biology
High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
 

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Article doi: 10.3791/2942
August 13th, 2011 •
1, 2
1Department Of Chemistry and Chemical Engineering, California Institute of Technology, 2Chemistry and Chemical Engineering, California Institute of Technology

Summary
Automatic Translation
August 13th, 2011

We describe a low cost, high throughput method to screen for fungal endoglucanase activity in E. coli. The method relies on a simple visual readout of substrate degradation, does not require enzyme purification, and is highly scalable. This allows for the rapid screening of large libraries of enzyme variants.

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