Functionalized Nanoparticle Targeting of Ovarian Cancer: A Technique to Facilitate Folic Acid-conjugated Nanoparticle Contrast Agent Uptake by Ovarian Cancer Cells

Folate receptors are membrane-associated glycoproteins overexpressed by a majority of ovarian cancer cells. Exploiting these receptors allows for the targeted delivery of diagnostic nanoparticles into the cancer cells.

Begin with a suspension of the desired folic acid-capped nanoparticle contrast agent conjugates. Add this nanoparticle suspension into an adherent culture of ovarian cancer cells. Incubate the culture for the desired time period.

The folic acid functionalized to the nanoparticles helps recognize the folate receptors expressed on the cancer cells, promoting the selective binding of nanoparticle conjugates with the cells. Subsequently, the plasma membrane invaginates, facilitating the cellular uptake and accumulation of nanoparticles.

Harvest the cancer cells. Next, centrifuge to separate the cells from the supernatant containing any unbound nanoparticles. Discard the supernatant and resuspend the nanoparticle-bearing cells in a suitable buffer for downstream applications.

First, mix the folic acid-capped copper sulfide nanoparticles at the proper concentration in fresh RPMI media. Add this nanoparticle solution to each well of the prepared 24-well plate that contains