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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Article DOI: 10.3791/3010-v • 07:48 min • September 22nd, 2011
September 22nd, 2011 •
1, 1, 1, 1, 1, 1, 1
1Merck Research Laboratory, Merck & Co., Inc

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A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line quality and high reproducibility.

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High-throughput Automated Platform Manufacturing Cell Lines Protein Therapeutics Biopharmaceutical Industry Production Systems Drug Supplies Manual Operations Labor-intensive Low-throughput Human Errors BD FACS Aria Cell Sorter CloneSelect Imager TECAN Freedom EVO Liquid Handling System Cell Line Development Process Transfected Cells Expression Vector Selection Agent Fluorescence-labeled Anti-human IgG Antibody Flow Cytometry Analysis Productive Cells Single-cell Sorting BD FACSAria Colony Formation Time-lapse Digital Images
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