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Pharmacological and Functional Genetic Assays to Manipulate Regeneration of the Planarian Dugesia japonica
Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
Journal (Biology)
Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis
Journal (Biology)
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Pharmacological and Functional Genetic Assays to Manipulate Regeneration of the Planarian Dugesia japonica

Article DOI: 10.3791/3058-v • 09:59 min • August 31st, 2011
August 31st, 2011 •
1, 1
1Department of Pharmacology and The Stem Cell Institute, University of Minnesota Medical School

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Summary
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An attractive model for studying stem cell differentiation within a live animal is the planarian flatworm. Regeneration is studied by simple amputation experiments that are easily performed in a basic laboratory and are amenable to pharmacological and genetic (in vivo RNAi) manipulation as detailed by protocols in this article.

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Planarian Dugesia Japonica Pharmacological Assays Functional Genetic Assays Regeneration Stem Cells Neoblasts Molecular Events Developmental Plasticity Neuroscientists Nervous System
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