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Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation
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Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

DOI:
10.3791/3087-v

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16:27 min

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September 14, 2011

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, ,
1Brigham and Women’s Hospital / Harvard Medical School,Department of Medicine, Cardiovascular Division, 2Weill Institute for Cell and Molecular Biology & Department of Biomedical Engineering,Cornell University

Chapters

  • 00:05Title
  • 01:47Preparation of Silicone Membrane Dishes and Adherence of Cells
  • 03:30Substrate Strain Experiments
  • 06:50Substrate Strain Analysis
  • 08:35Representative Results for Substrate Strain Application on the Interphase Cell Nucleus
  • 09:57Microneedle Manipulation Experiment
  • 12:19Microneedle Manipulation Analysis
  • 13:50Representative Results for Microneedle Manipulation of the Interphase Cell Nucleus
  • 15:58Conclusion

Summary

Automatic Translation

Automatic Translation

We present two independent, microscope-based tools to measure the induced nuclear and cytoskeletal deformations in single, living adherent cells in response to global or localized strain application. These techniques are used to determine nuclear stiffness (i.e., deformability) and to probe intracellular force transmission between the nucleus and the cytoskeleton.

Tags

Biophysical AssaysMechanical PropertiesInterphase Cell NucleusSubstrate Strain ApplicationMicroneedle ManipulationEukaryotic CellsNucleus StiffnessCytoskeletonBiomechanical BehaviorPhysiological ConditionsPathological ConditionsMigrating NeutrophilsInvading Cancer CellsExtravasationNarrow SpacesMuscleStructural SupportRepetitive Mechanical StressCellular ArchitectureNuclear Envelope ProteinsLaminsNesprinsNucleo-cytoskeletal CouplingHuman Diseases

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