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Biology

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Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus
 

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

Article doi: 10.3791/3087
September 14th, 2011

Summary September 14th, 2011

We present two independent, microscope-based tools to measure the induced nuclear and cytoskeletal deformations in single, living adherent cells in response to global or localized strain application. These techniques are used to determine nuclear stiffness (i.e., deformability) and to probe intracellular force transmission between the nucleus and the cytoskeleton.

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