Antibody Production from a Batch Overgrowth Culture of Transfected Mammalian Cells

Begin with a flask containing stably transfected mammalian cells in a serum-free medium supplemented with a non-ionic surfactant to prevent cell aggregation.

These cells carry a modified genome containing integrated heavy-and-light-chain genes for therapeutic antibody production.

Introduce tryptone, a nutrient-rich source of peptides and amino acids essential for cellular metabolism.

The cell utilizes these amino acids and peptides, and multiplies, resulting in an overgrowth that supports a higher antibody yield.

The expression of genes in the cells drives the production of heavy and light chain polypeptides.

In the endoplasmic reticulum, these chains fold and dimerize via inter-chain disulfide bonds, forming therapeutic antibodies.

In the Golgi apparatus, antibodies undergo post-translational modifications. They are then packaged into vesicles and released into the medium.

Incubate the cells for a longer duration to increase antibody production.

As cell viability begins to decline, harvest the supernatant.

Filter the supernatant containing therapeutic antibodies and store it at a lower temperature for later use.

Seed cells at 2 x 10⁵ cells per milliliter in 100 milliliters of seru