Evaluating the Effects of Fecal Microbiota Transplantation in an Interleukin-10 Deficient Mouse

Begin with a restrained mouse lacking interleukin-10, an anti-inflammatory cytokine, which results in colon inflammation.

Take a syringe filled with a fecal suspension rich in diverse gut microbes, from a healthy mouse.

Next, attach an oral tube to the syringe.

Insert this tube into the mouth, guiding it downward to the stomach of the mouse.

Now, administer the fecal suspension directly into the stomach, allowing it to gradually reach the colon.

Once in the colon, the fecal microbes multiply and colonize, reestablishing the gut microbes.

The colonized microbes then release various secondary metabolites.

These metabolites help in mucin production, which repairs the colon epithelium and restores the gut integrity.

Within the colon epithelium, the fecal microbes and their metabolites interact with immune cells.

This interaction causes the immune cells to release anti-inflammatory cytokines, leading to a reduction in colon inflammation in the interleukin-10 deficient mouse.

Prepare a sterile solution by adding 10% glycerol solution to normal saline. Add 10 milliliters of this solution to the conical tube for each gram of fecal pellets. Homogenize this mixture at a low speed three times