Establishing an Alzheimer's Disease Model in Zebrafish Brain Through Amyloid Peptide Injection

Fill a glass injection capillary with a solution of synthetic monomeric amyloid peptides, which are abnormal peptides with aggregation-prone properties.

Insert the capillary into a microinjection holder.

Simultaneously place a zebrafish in a petri dish containing an anesthetic solution.

Once the fish is anesthetized, hold it laterally with forceps to secure the head.

Make a small incision in the fish skull at the junction of the two lateral plates to access the neuron-rich forebrain.

Now, insert the capillary needle through the incision, and inject the amyloid peptide solution into the forebrain.

Transfer the zebrafish to a recovery container with circulating water.

Inside the forebrain, the amyloid peptides accumulate near the neurons.

These peptides spontaneously misfold to form beta-sheets and aggregate around the neurons to form toxic amyloid plaques.

These plaques damage the neurons and impair their signal transmission.

This establishes a zebrafish model of Alzheimer's disease, a brain disorder characterized by amyloid plaques.

Use a needle puller with parameters outlined in the text protocol, to prepare glass injection capillaries. Adjust the pressure setting on the pressure