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Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

DOI:
10.3791/3143-v

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09:02 min

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November 11, 2011

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1Membrane Biology Group, Centre for integrative Physiology,University of Edinburgh

Chapters

  • 00:05Title
  • 02:02Experimental Setup
  • 02:36Sample Preparation
  • 04:32S1 Phase
  • 06:12Data Analysis

Summary

Automatic Translation

Automatic Translation

A live fluorescence imaging technique to quantify the replenishment and mobilisation of specific synaptic vesicle (SV) pools in central nerve terminals is described. Two rounds of SV recycling are monitored in the same nerve terminals providing an internal control.

Tags

Quantitative AnalysisSynaptic Vesicle Pool ReplenishmentCultured Cerebellar Granule NeuronsFM DyesNeurotransmitter ReleaseEndocytosisRecycling PoolReadily Releasable PoolReserve PoolAssaySV TrafficClathrin-dependent EndocytosisActivity-dependent Bulk EndocytosisMobilisation MechanismsSV TurnoverCentral Nerve TerminalsHydrophobic Hydrocarbon TailHydrophilic Head GroupFluorescent Probes

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