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Visualizing Dengue Virus through Alexa Fluor Labeling
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Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Dengue Virus through Alexa Fluor Labeling

Visualizing Dengue Virus through Alexa Fluor Labeling

DOI:
10.3791/3168-v

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09:11 min

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July 09, 2011

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, ,
1Defence Medical and Environmental Research Institute,DSO National Laboratories, 2Program in Emerging Infectious Diseases,Duke-NUS Graduate Medical School, 3Program in Emerging Infectious Diseases,Duke-NUS Graduate Medical School

Chapters

  • 00:05Title
  • 01:20Alexa Fluor Labeling of Dengue Virus
  • 03:39Assessing Viability and Degree of Labeling

Summary

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Taking advantage of the advancements in fluorophore development and imaging technology, a simple method of Alexa Fluor labeling of dengue virus was devised to visualize the early interactions between virus and cell.

Tags

VisualizingDengue VirusAlexa Fluor LabelingInteractionVirus And CellInfectionDisease PathogenesisVaccineTherapeutic DesignFluorophores DevelopmentImaging TechnologyDengue PathogenesisDengue InfectionsEnveloped Dengue VirusEnvelope GlycoproteinNucleocapsid ShellRNA GenomeAmine Reactive DyeImmunofluorescent MicroscopyLabeling MethodAlexa Fluor Succinimidyl Ester DyeSodium Bicarbonate BufferLive Virus Labeling

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