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In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model
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In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model

In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model

DOI:
10.3791/3175-v

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11:02 min

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October 03, 2011

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, , , , , ,
1Department of Radiation Oncology,University of Texas Southwestern Medical Center , 2Department of Radiology,University of Texas Southwestern Medical Center , 3Department of Radiation Oncology,Kyoto University Graduate School of Medicine

Chapters

  • 00:05Title
  • 01:46Validation of MDA-MB231/5HRE-ODD-luc Cells as a Tool for Measuring Hypoxia Dynamics
  • 04:03Culture of MDA-MB231/5HRE-ODD-luc Cells for Surgical Implantation
  • 04:36Surgical Implantation of MDA-MB231/5HRE-ODD-luc Cells into the Mouse Brain
  • 06:23In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics in Breast Cancer Brain Metastasis
  • 08:23Representative Results In vitro and In vivo Bioluminescence Assays
  • 10:35Conclusion

Summary

Automatic Translation

Automatic Translation

Bioluminescence imaging of hypoxia inducible factor-1α activity is applied to monitor intracranial tumor hypoxia development in a breast cancer brain metastasis mouse model.

Tags

In Vivo Bioluminescence ImagingTumor Hypoxia DynamicsBreast Cancer Brain MetastasisMouse ModelMalignant ProgressionTherapeutic ResponseIntracranial DevelopmentOrthotopic TumorsBioluminescence Imaging (BLI)Luciferase Gene ExpressionCancer ResearchTumor GrowthTreatment ResponseReporter GeneHypoxia Inducible Factor-1α (HIF-1α)Gene TranscriptionMDA-MB231 CellsHIF-1α Bioluminescence AssayHypoxic ChamberPhoton Flux

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