JoVE Encyclopedia of Experiments
Neuroscience
0 views • 6:28 min • August 7th, 2025
Begin with an anesthetized rat with screws embedded in its skull.
The rat has a chest-implanted counter electrode with a subcutaneously tunneled cable secured around one of the skull-embedded screws.
Solder the cable to a connector.
Glue a transcranial electrical brain stimulation or tES electrode socket onto the skull above the motor cortex.
Seal the assembly with glue, then secure it with dental cement.
Reposition the skin and allow the rat to recover.
Fill the socket with saline.
Screw the tES electrode cap containing the stimulation electrode.
Connect a stimulation device to the connectors.
Place the rat in a cage that allows free movement.
Initiate anodal stimulation to generate an electric field in the motor cortex, enabling voltage-gated ion channel opening, positive ion influx, and neuron depolarization.
This enhances synaptic activity, triggering ATP release, which activates microglia, crucial for immune responses.
Neuronal and microglial activation highlights the neurotherapeutic potential of tES.
Begin by scraping off the periosteum, the connective tissue on the skull, to the sides with the scalpel, and thoroughly wipe off with cotton swabs. Fixate the connective tissue at the four
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