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Journal / Biology / Purification de Hsp104, une protéine Disaggregase
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Purification de Hsp104, une protéine Disaggregase

Article DOI: 10.3791/3190-v • 07:18 min • September 30th, 2011
September 30th, 2011 •
1, 1, 1
1Department of Biochemistry and Biophysics, University of Pennsylvania

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Ici, nous décrivons un protocole pour la purification de la très active Hsp104, un AAA + hexamérique protéines de la levure, qui couple l'hydrolyse d'ATP à la désagrégation des protéines. Ce schéma exploite une construction His6-marqués pour la purification d'affinité à partir

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Biologie Moléculaire Numéro 55 neurosciences Hsp104 AAA + disaggregase choc thermique amyloïde prion
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