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Journal / Biology / Очистка Hsp104, белка Disaggregase
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Очистка Hsp104, белка Disaggregase
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Очистка Hsp104, белка Disaggregase

Article DOI: 10.3791/3190-v • 07:18 min • September 30th, 2011
September 30th, 2011 •
1, 1, 1
1Department of Biochemistry and Biophysics, University of Pennsylvania

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Здесь мы опишем протокол для очистки высокоактивной Hsp104, hexameric AAA + белка из дрожжей, которые пары гидролиза АТФ с белком дезагрегации. Эта схема использует His6 с метками построить для аффинной очистки от

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Молекулярная биология выпуск 55 неврологии Hsp104 AAA + disaggregase тепловой шок амилоида прион
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