Twin-to-twin transfusion syndrome and twin anemia polycythemia sequence are two potentially devastating problems in perinatal medicine. Both disorders occur only in monochorionic twins and result from unbalanced blood flow through placental vascular anastomoses. We provide a simple protocol to accurately evaluate the presence of vascular anastomoses using colored dye injection of placental vessels after birth.
Lopriore, E., Slaghekke, F., Middeldorp, J. M., Klumper, F. J., van Lith, J. M., Walther, F. J., Oepkes, D. Accurate and Simple Evaluation of Vascular Anastomoses in Monochorionic Placenta using Colored Dye. J. Vis. Exp. (55), e3208, doi:10.3791/3208 (2011).