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Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats
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Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats

Article DOI: 10.3791/325 • 22:07 min • October 1st, 2007
October 1st, 2007 •
1, 1
1Department of Physiology and Biophysics, University of California, Irvine (UCI)

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Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T (TEM) lymphocytes. Here we demonstrate how to activate antigen-specific TEM cells, induce adoptive DTH in Lewis rats and monitor the inflammatory response.

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Induction Monitoring Adoptive Delayed-type Hypersensitivity Rats DTH Inflammatory Reaction CCR7- Effector Memory T Lymphocytes Antigen Immune System Immunosuppressants Cutaneous DTH Adoptive Transfer Active Immunization Intradermal Challenge Ovalbumin Tuberculin Tetanus Toxoid Keyhole Limpet Hemocyanin Autoantigen Myelin Basic Protein (MBP) Experimental Autoimmune Encephalomyelitis (EAE) Lewis Rats Stimulation Ovalbumin-specific T Cells Intraperitoneally Injection Equilibrate In Vivo Pinna Saline 3-72 Hours Later
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