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JoVE Journal
Biology

This content is Open Access.

A Protocol for Computer-Based Protein Structure and Function Prediction
 

A Protocol for Computer-Based Protein Structure and Function Prediction

Article DOI: 10.3791/3259
November 3rd, 2011

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Summary November 3rd, 2011

Guidelines for computer based structural and functional characterization of protein using the I-TASSER pipeline is described. Starting from query protein sequence, 3D models are generated using multiple threading alignments and iterative structural assembly simulations. Functional inferences are thereafter drawn based on matches to proteins with known structure and functions.

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