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A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
This content is Free Access.
Chapters
- 00:05Title
- 01:33Preparatory Dissection of Mosquito Specimens
- 02:28DNA Extraction from Mosquito Specimens
- 04:03Plasmodium falciparum Genotyping and Anopheles spp. Molecular Identification
- 05:25Representative PCR Assay Results
- 06:55Conclusion
A rapid and affordable way to extract quality malaria parasite and vector DNA from mosquito specimens is described. Capitalizing on chelating properties of Chelex resin, the simple method enables genotyping of malaria parasites in mosquito mid-gut and salivary gland phases, as well as molecular identification of the Anopheles sibling species by PCR.
Tags
DNA ExtractionChelex ProtocolAnopheles Spp.Molecular ToolsMalaria ParasitesVector MosquitoesControl ProgramsOperations ResearchField Collected Mosquito SpecimensAnopheles Gambiae Sl.Microcentrifuge TubesHomogenizationDeionized WaterDNA Extraction MethodsChelex-based TechniqueStandard Salting Out Extraction
