A Simple Chelex Protocol for DNA Extraction from <em>Anopheles</em> spp.

Mulenga Musapa; Taida Kumwenda; Mtawa Mkulama; Sandra Chishimba; Douglas E. Norris; Philip E. Thuma; Sungano Mharakurwa

doi:10.3791/3281

Journal

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Immunology and Infection

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A Simple Chelex Protocol for DNA Extraction from Anopheles spp.

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.

54,631 Views

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07:16 min

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January 09, 2013

DOI:

10.3791/3281-v

DOI:

10.3791/3281-v

•

07:16 min

January 09, 2013

•

53 Views

Mulenga Musapa¹, Taida Kumwenda¹, Mtawa Mkulama¹, Sandra Chishimba¹, Douglas E. Norris², Philip E. Thuma¹, Sungano Mharakurwa¹

¹Malaria Institute at Macha, ²Department of Molecular Microbiology & Immunology,Johns Hopkins Bloomberg School of Public Health

Summary

A rapid and affordable way to extract quality malaria parasite and vector DNA from mosquito specimens is described. Capitalizing on chelating properties of Chelex resin, the simple method enables genotyping of malaria parasites in mosquito mid-gut and salivary gland phases, as well as molecular identification of the Anopheles sibling species by PCR.