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JoVE Journal
Immunology and Infection

This content is Open Access.

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
 

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.

Article DOI: 10.3791/3281
January 9th, 2013

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Summary January 9th, 2013

A rapid and affordable way to extract quality malaria parasite and vector DNA from mosquito specimens is described. Capitalizing on chelating properties of Chelex resin, the simple method enables genotyping of malaria parasites in mosquito mid-gut and salivary gland phases, as well as molecular identification of the Anopheles sibling species by PCR.

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