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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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Biology
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JoVE Journal Biology
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

DOI:
10.3791/3303-v

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08:40 min

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April 06, 2012

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, , , , ,
1Sigma-Aldrich

Chapters

  • 00:05Title
  • 01:39Transfection with Target ID Library and Selection for Stable Cell Lines
  • 03:59Selection Reagent Kill Curves
  • 04:52Transfection of Library Cells with miRNA Expression Constructs and Selection for Stable Cell Lines and miRNA Targets
  • 06:53Library Screen for miR-373 Targets
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

The Target ID Library is a plasmid-based, genome-wide collection of cloned cDNA used to identify miRNA targets. Here we demonstrate its use and application.

Tags

Genome-wide ScreenMiRNA TargetsMISSION Target ID LibraryPlasmid-based LibraryCDNA LibraryThymidine Kinase-zeocinStable TransformantsMiRNA Of InterestCDNAs Containing MiRNA’s TargetSequencingHuman TranscriptomeOligo-dT PrimingTotal RNASigma-Aldrich WebpageIllumina SequencingUnique GenesUCSC RefGene

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