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Chapters
- 00:05Title
- 01:36Initiating the ZFN Design Process
- 03:23Custom ZFN Production and Validation
- 04:16Delivery of Validated ZFNs by Nucleofection
- 06:25Cel-1 Assay
- 07:09Clonal Isolation and Characterization
- 07:46Estimated ZFN Activity from the Cel-1 Assay
- 08:27Conclusion
CompoZr Özel Çinko-Parmak Nükleaz (ZFN) Hizmet kullanıcı tarafından tanımlanan herhangi bir odağı herhangi bir organizma ya da hücre hattı hassas genom düzenlenmesini sağlar. Bu makale CompoZr Özel ZFN Hizmet tasarım, imalat, onaylanması ve uygulanması için süreci anlatılmaktadır.
Tags
Genome EditingCompoZr Custom Zinc Finger NucleasesGene FunctionGenetic Basis Of DiseaseTraditional Gene Editing MethodsChemical-based MutagenesisRandom Integration Of DNA SequencesSynthetic SequencesAberrant Culture ConditionsTransient ZFN ExpressionPrecise Gene EditingHighly Efficient MannerZinc Finger NucleasesDouble-stranded DNA (dsDNA)CompoZr ZFN UnitDNA-cleavage DomainsDouble-strand Break (DSB)Non-homologous End-joining (NHEJ) DNA Repair PathwaySmall Insertions And Deletions (indels)FrameshiftFunctional Knockout
