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成像遥感信息素在小鼠犁鼻急性组织切片的制备
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation
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成像遥感信息素在小鼠犁鼻急性组织切片的制备

DOI:
10.3791/3311-v

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09:31 min

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December 06, 2011

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, , , ,
1Department of Pharmacology and Toxicology,University of Lausanne, 2Department of Genetics and Evolution,University of Geneva

Chapters

  • 00:05Title
  • 02:01Dissection of the Mouse Vomeronasal Organ (VNO)
  • 03:37VNO Tissue Slice Preparation
  • 04:37Immunohistochemistry of VNO Floating Slices
  • 05:19Calcium Imaging of VNO Slices
  • 06:23Representative Immunostaining and Calcium Imaging of VNO Slice
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

在小鼠体内，能够检测信息素主要介导犁鼻器（VNO）。在这里，描述一种急性组织VNO切片进行钙成像的准备。这种生理方法允许生活在一个组织中的亚群和/或单个神经元的观察和受体 - 配体识别的方便。

Tags

Pheromone SensingMousePheromonesIntra-species CommunicationBiological FluidsUrinePheromonal CommunicationKin InteractionsHierarchical OrganizationsSexual InteractionsSurvival Of SpeciesVomeronasal Organ (VNO)Nasal CavityCartilaginous CapsuleSensory NeuroepitheliumVomeronasal Bipolar Sensory Neurons (VSNs)DendriteMicrovilliChemoreceptorLigandsVomeronasal Receptor FamiliesV1RsV2Rs

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