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Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay
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Neuroscience
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JoVE Journal Neuroscience
Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay

Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay

DOI:
10.3791/3330-v

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12:17 min

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January 19, 2012

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, ,
1Department of Biology,California State University, Northridge, 2Centro de Biología Celular y Molecular,Universidad Nacional de Córdoba

Chapters

  • 00:05Title
  • 01:55Isolation of Trunk Neural Tubes for Overnight Culture on Coverslips
  • 06:05Loading the Modified Zigmond Chamber and Time-lapse Analysis of Cell Migration
  • 09:50Representative Results
  • 11:50Conclusion

Summary

Automatic Translation

Automatic Translation

An approach to analyze the migration of explanted cells (trunk neural crest cells) is described. This method is inexpensive, gentle, and capable of distinguishing chemotaxis from both chemokinesis and other influences on migratory polarity such as those derived from cell-cell interactions within the primary trunk neural crest cell culture.

Tags

Trunk Neural Crest Cell MigrationZigmond Chamber AssayEpithelial-mesenchymal TransitionTarget RecognitionNCC DifferentiationGuidance MechanismsNegative Guidance CuesChemoattractantsIn Vitro ApproachesPrimary Stem Cell Cultures

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