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Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
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Chapters
- 00:05Title
- 01:39Construction of a Laser Ablation System
- 03:42Aligning the Laser Beam
- 04:54Expanding and Focusing the Laser Beam
- 08:29Axotomies using the Assembled System
- 09:21Conclusion
Laser axotomy followed by time-lapse imaging is a sensitive way to assay the effects of mutations in C. elegans on axon regeneration. A high quality, but inexpensive, laser ablation system can be easily added to most microscopes. Time lapse imaging over 15 hours requires careful immobilization of the worm.
Tags
Low-budgetLaser Axotomy SystemStudyAxon RegenerationC. ElegansAssayCostTechnical ExpertiseLaser Ablation SystemSolid-state Pulse LasersTransparent PreparationsConstructionAlignmentOptical PathFocused CondenserIntermediate ModuleDichroicImagingLaser BeamMicroscope Condenser LensObjective LensFinal AlignmentTesting
