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JoVE Journal
Bioengineering

This content is Open Access.

Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
 

Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans

Article DOI: 10.3791/3331
November 15th, 2011

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Summary November 15th, 2011

Laser axotomy followed by time-lapse imaging is a sensitive way to assay the effects of mutations in C. elegans on axon regeneration. A high quality, but inexpensive, laser ablation system can be easily added to most microscopes. Time lapse imaging over 15 hours requires careful immobilization of the worm.

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