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Spheroid Assay to Measure TGF-β-induced Invasion
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Spheroid Assay to Measure TGF-β-induced Invasion

Article DOI: 10.3791/3337
November 16th, 2011 •
1, 1, 1, 1
1Department of Molecular Cell Biology and Centre for Biomedial Genetics, Leiden University Medical Centre

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November 16th, 2011

An assay to quantitatively measure Transforming Growth Factor (TGF)-β-induced invasion in 3-dimensional collagen gels is described. This assay takes advantage of the MCF10A series of cell lines, which represent different stages of breast cancer development. This method can be adopted to be used with other cell lines and might be used to investigate other potential activators or inhibitors of invasion.

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