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Spheroid Assay zur TGF-β-induzierte Invasion Measure

Article DOI: 10.3791/3337 • 09:19 min
November 16th, 2011 •
1, 1, 1, 1
1Department of Molecular Cell Biology and Centre for Biomedial Genetics, Leiden University Medical Centre

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November 16th, 2011

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Ein Test zur quantitativen Messung Transforming Growth Factor (TGF)-β-induzierte Invasion im 3-dimensionalen Kollagen-Gelen beschrieben. Dieser Assay nutzt die MCF10A Reihe von Zelllinien, die verschiedenen Stadien der Entwicklung von Brustkrebs darstellen. Diese Methode kann angenommen, um mit anderen Zelllinien verwendet werden und könnte verwendet werden, um andere potenzielle Aktivatoren oder Inhibitoren der Invasion zu untersuchen sein.

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