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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
 

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Article DOI: 10.3791/3410
January 27th, 2012

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Summary January 27th, 2012

FRET-based reporters are increasingly used to monitor kinase and phosphatase activities in live cells. Here we describe a method on how to use FRET-based reporters to assess cell cycle-dependent changes in target phosphorylation.

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