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1Department of Physiology and Biophysics, Confocal Microscopy and Cell Imaging Core,Robert Wood Johnson Medical School , 2Department of Physiology and Biophysics,Robert Wood Johnson Medical School , 3Muscle Biology Research Group-MUBIG Schools of Nursing & Medicine,University of Missouri-Kansas City
Chapters
- 00:05Title
- 02:11Intracellular Ca2+ Measurement for Individual Cells
- 04:45Mn2+ Quenching Assay in Cultured Cells
- 05:20Mn2+ Quenching Assay in Muscle Cells
- 06:40Spatially and Temporally Resolved SOCE in Skinned Muscle Fibers
- 10:20SOCE Activity in KYSE-150 and Muscle Cells
The extent of store-operated Ca2+ entry (SOCE) can be monitored using fluorescent Ca2+ indicators. Mn2+ quenching of such indicators assays SOCE in cultured cells and skeletal muscle fibers. A technique allowing spatial and temporal resolution of SOCE by confocal imaging of mechanically skinned muscle fibers is also described.
Tags
Fluorescence-based MeasurementStore-operated Calcium EntryLive CellsCultured Cancer CellSkeletal Muscle FiberSOCECapacitative Ca2+ EntryEndoplasmic ReticulumSarcoplasmic ReticulumSecond MessengerCellular ProcessesProliferationApoptosisGene TranscriptionMotilityEpithelial CellsPatch-clamp StudiesIntracellular Ca2+ MeasurementsHigh-throughput ScreeningFluorescence Methods
