Manganese Quenching Assay
Manganese Quenching Assay - Related Videos
10:57
Quenching and Boiling
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2023
Source: Alexander S Rattner, Sanjay Adhikari, and Mahdi Nabil; Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA Controlled heating followed by rapid cooling is an important element of many materials processing applications. This heat-treating procedure can increase material hardness, which is important for cutting tools or surfaces in high wear environments. The rapid cooling stage is called quenching, and is often performed by immersing...
10:07
Improving Thermoelectric Properties of Bi2Te3 Thin Films By Manganese Co-Sputtering
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2026
A radiofrequency co-sputtering protocol was developed to fabricate manganese-doped Bi2Te3 thin films and evaluate how manganese input influences structural and thermoelectric transport properties. Moderate manganese incorporation improved film uniformity and power-factor-related transport behavior, while higher manganese input increased structural disorder and resistivity.
09:02
Manganese Oxide Nanoparticle Synthesis by Thermal Decomposition of Manganese(II) Acetylacetonate
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Cited by 7 •
2020
This protocol details a facile, one-pot synthesis of manganese oxide (MnO) nanoparticles by thermal decomposition of manganese(II) acetylacetonate in the presence of oleylamine and dibenzyl ether. MnO nanoparticles have been utilized in diverse applications including magnetic resonance imaging, biosensing, catalysis, batteries, and waste water treatment.
08:36
Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
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Cited by 4 •
2012
A technique is described for broadly opening the blood-brain barrier in the mouse using microbubbles and ultrasound. Using this technique, manganese can be administered to the mouse brain. Because manganese is an MRI contrast agent that accumulates in depolarized neurons, this approach enables imaging of neuronal activity.
07:47
Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
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Cited by 7 •
2016
Quorum-quenching enzymes are anti-virulent and anti-bacterial options that can mitigate pathogenesis without risk of incurring resistance, by preventing the expression of virulence factors and genes associated with antibiotic resistance and biofilm formation. In this study, we report a method that demonstrates the efficacy of quorum-quenching enzymes in bacterial biofilm disruption.
FAQs
The assay tracks fluorescence loss caused by Mn2+ entering through pathways that also allow Ca2+ influx. Because the signal reflects quenching of the intracellular indicator rather than a rise in intracellular calcium, it is largely independent of calcium released from internal stores. This distinction helps identify plasma-membrane entry even when calcium mobilization occurs simultaneously.
Fura-2 serves as the intracellular fluorescent indicator whose signal decreases when entering Mn2+ quenches its fluorescence. Monitoring this decline provides a time-dependent readout of divalent cation entry. The indicator therefore converts channel-permeability activity into an optical measurement, allowing researchers to follow influx without relying solely on changes in intracellular calcium concentration.
The rate of fluorescence decline provides an estimate of how quickly Mn2+ enters the cell through relevant permeable pathways. A faster loss indicates more rapid entry under the tested conditions, whereas a slower loss indicates reduced entry. This kinetic readout is useful for comparing channel activity across experimental manipulations rather than recording only a final fluorescence value.
Conventional calcium imaging can combine calcium entering across the plasma membrane with calcium released from intracellular stores, making the sources difficult to distinguish. Mn2+ quenching adds a separate influx-focused readout because fluorescence loss follows entry through calcium-permeable pathways. Using the approaches as complementary measurements can clarify whether an observed calcium signal reflects membrane influx, internal release, or both.
Living cells are monitored with an intracellular fluorescent indicator, such as fura-2, while extracellular Mn2+ is available to enter through calcium-permeable pathways. Researchers follow the resulting fluorescence decrease over time and interpret its rate as an entry measurement. The workflow can then be applied under different channel, pharmacological, or genetic conditions to compare influx.
This method is particularly useful when investigators need to evaluate plasma-membrane channel function or store-operated calcium entry. It can also test how pharmacological or genetic manipulation changes divalent cation influx. In these settings, the assay supplies an influx-specific outcome that strengthens interpretation of experiments where standard calcium measurements cannot resolve entry from intracellular calcium release.