Microfluidic Chip Fabrication
Microfluidic Chip Fabrication - Related Videos
09:43
Microfluidic Chip Fabrication and Method to Detect Influenza
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Cited by 6 •
2013
An integrated microfluidic thermoplastic chip has been developed for use as a molecular diagnostic. The chip performs nucleic acid extraction, reverse transcriptase, and PCR. Methods for fabricating and running the chip are described.
11:16
A Microfluidic Chip for ICPMS Sample Introduction
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Cited by 4 •
2015
We present a discrete droplet sample introduction system for inductively coupled plasma mass spectrometry (ICPMS). It is based on a cheap and disposable microfluidic chip that generates highly monodisperse droplets in a size range of 40−60 µm at frequencies from 90 to 7,000 Hz.
16:00
Fabrication of the Thermoplastic Microfluidic Channels
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2008
Here we demonstrate how to fabricate thermoplastic microfluidic chips using hot embossing and heat sealing. Then we demonstrate how to use in situ light directed surface grafting and polymerization through the sealed chip to form the composite solid phase columns.
13:42
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
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Cited by 9 •
2017
A microfluidic biosensor platform was designed and fabricated using low-cost dry film photoresist technology for the rapid and sensitive quantification of various analytes. This single-use system allows for the electrochemical readout of on-chip-immobilized enzyme-linked assays by means of the stop-flow technique.
15:41
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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Cited by 10 •
2013
In this article we present a microfluidic chip for single cell analysis. It allows the quantification of intracellular proteins, enzymes, cofactors, and second messengers by means of fluorescent assays or...
FAQs
Photolithography first creates a patterned mold that defines the intended channel geometry. Soft lithography then uses that mold to replicate the design in PDMS, transferring the microscale pattern into a polymer layer. This sequence separates design definition from device replication, allowing the same patterned architecture to support controlled fluid handling in subsequent bioengineering experiments.
Plasma bonding seals patterned PDMS to glass or another substrate by joining the device layers into an enclosed structure. This step is essential because open channels cannot maintain the controlled fluid paths required for analysis, mixing, separation, or detection. The selected substrate also contributes to the device's compatibility with biological systems.
PDMS can serve as the polymer used to replicate a patterned channel design, while glass or another substrate can provide the surface for sealing. Material selection matters because ongoing advances in materials and manufacturing aim to improve device robustness, scalability, and compatibility with biological systems. These considerations help align fabrication choices with bioengineering use.
Channel geometry and device integration shape what the platform can do. Precisely engineered channels can be organized for fluid handling, mixing, separation, and detection, while the small operating scale supports analysis with limited samples and reagents. Designing these functions into one compact device helps connect fabrication decisions to the intended experimental or diagnostic outcome.
A typical workflow begins by using photolithography to pattern a mold, followed by soft lithography to replicate the pattern in PDMS. The patterned polymer layer is then sealed to glass or another substrate through plasma bonding. Together, these stages convert a designed channel layout into an enclosed platform suitable for controlled bioengineering analyses.
Bioengineers can apply these chips to cell culture, biochemical assays, tissue models, and point-of-care diagnostics. The same compact platform can integrate fluid handling with mixing, separation, and detection, allowing the fabrication strategy to support different biological tasks. The intended application determines which channel functions and material choices should be incorporated into the device.
Working at microliter- to nanoliter-scale volumes enables controlled analysis with small samples and reagent quantities. This capability is particularly relevant when experiments involve limited biological material or require several fluid operations in a compact platform. Integrating handling, mixing, separation, and detection can further connect volume control with practical assay and diagnostic workflows.