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JoVE Journal
Biology

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Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
 

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

Article DOI: 10.3791/3448
February 27th, 2012

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Summary February 27th, 2012

We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C. elegans.

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