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JoVE Journal Bioengineering
Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture
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Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture

Article DOI: 10.3791/3490 • 13:37 min • February 2nd, 2012
February 2nd, 2012 •
1, 1
1ARTORG Center for Biomedical Engineering, University of Bern

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Summary
Automatic Translation

This protocol illustrates a harvesting technique for coccygeal bovine intervertebral discs for organ culture for in vitro organ culture.

Tags

Intact Bovine Tail Intervertebral Discs Organ Culture Intervertebral Disc (IVD) Spine Nucleus Pulposus (NP) Annulus Fibrosus (AF) Cartilaginous Endplates Degenerative Intervertebral Disc Disease (DDD) Disc Herniations Discogenic Pain In Vitro Organ Culture Live Disc Cells Mechano-biological Aspects Bovine Coccygeal IVDs Human Lumbar IVDs Cell Density Cell Population Dimensions Bovine Caudal IVD Harvesting Techniques Cartilaginous Endplates Bony Endplates Avascular Organs Diffusion Capillary Buds
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