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Journal
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Immunology and Infection
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2光子顕微鏡を用いてマウスの胸腺の生体内イメージング
JoVE Journal
Immunology and Infection
Author Produced
This content is Free Access.
JoVE Journal
Immunology and Infection
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
2光子顕微鏡を用いてマウスの胸腺の生体内イメージング
DOI:
10.3791/3504-v
•
10:08 min
•
January 07, 2012
•
Susana S. Caetano
,
Tatiana Teixeira
,
Carlos E. Tadokoro
1
Laboratory of Immune Regulation
,
Instituto Gulbenkian de Ciência
Chapters
00:01
Title
00:05
Introduction
01:15
Animal preparation: Irradiation and bone marrow transfer
03:16
Animal preparation: Thymic transplantation
04:58
Animal preparation: Surgery to exposure transplanted thymus
06:53
Two-photon image acquisition
08:35
Results
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々は、胸腺の生体内イメージング買収のための新たな研究ツールとプロトコルを開発しました。我々の技術は、T細胞の開発が発生した胸腺内で"ニッチ"の同定に役立つはずです。
Tags
Intravital Imaging
Mouse Thymus
2-photon Microscopy
Image Acquisition
Organs
Stereotactic Tools
Surgical Procedures
Cellular Behaviors
Live Animals
Ex Vivo Imaging Acquisition
Surgical Procedure
Organ Holder
Foxp3+ Cells
Regulatory T Cells
Naturally-occurring Tregs (nTregs)
Peripherally-converted Tregs (pTregs)
In Vivo Interactions
Phenotype And Physiology
Nude Mice
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