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Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS
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Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS

Article DOI: 10.3791/3511-v • 07:33 min • December 21st, 2011
December 21st, 2011 •
1, 1, 2, 1
1Department of Biochemistry, Temple University, 2Department of Anesthesiology and Pain Medicine, University of Washington

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Summary
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An efficient method to gain insights into visualizing the paracrine-derived ROS induction of endothelial Ca2+ signaling is described. This method takes advantage of measuring paracrine derived ROS triggered Ca2+ mobilization in vascular endothelial cells in a co-culture model.

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Vascular Ca2+ Signaling Paracrine ROS (reactive Oxygen Species) Oxidative Stress Ischemia/reperfusion Damage Sepsis Signal Transduction Cell Proliferation Cell Death Tissue Damage Inflammatory Cells Cytokines Tumor Necrosis Factor-alpha (TNF-alpha) IL-1 Endothelial Cells (EC) Endothelial Dysfunction Macrophages NADPH Oxidases
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