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JoVE Journal
Immunology and Infection

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Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
 

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Article DOI: 10.3791/3547
April 9th, 2012

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Summary April 9th, 2012

The Xpert MTB/RIF test integrates sample decontamination, hands-free operation, on-board sample processing, and ultra-sensitive hemi-nested PCR for the simultaneous detection of Mycobacterium tuberculosis and rifampicin resistance, either in expectorated sputum or concentrated sputum sediments, in approximately two hours. Testing is standardized and requires only moderate laboratory infrastructure and training.

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