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Chapters
- 00:05Title
- 01:37Preparation of Large Unimellar Lipid Vesicles (LUVs)
- 05:01Quantifying LUV Concentration
- 08:05Preparing Peptide Solutions
- 08:46Translocation Quantification
- 10:37Results: Translocation Assay
- 11:58Conclusion
This protocol details a method for the quantitative measure of peptide translocation into large unilamellar lipid vesicles. This method also provides information about the rate of membrane translocation and can be used to identify peptides that efficiently and spontaneously cross lipid bilayers.
