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Immunology and Infection

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Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus

Article DOI: 10.3791/3627-v • 11:18 min • May 7th, 2012
May 7th, 2012 •
1, 1,2, 3, 1,2,4,5
1Center for Cell and Gene Therapy, Baylor College of Medicine, 2Pathology and Immunology, Baylor College of Medicine, 3Department of Stem Cell Transplantation and Cellular Therapy, University of Texas M.D. Anderson Cancer Center, 4Medicine, Baylor College of Medicine, 5Department of Pediatrics, Baylor College of Medicine

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Summary
Automatic Translation

Here we describe the first good manufacturing practice (GMP)-compliant method of producing virus-specific cytotoxic T lymphocytes (CTL) from umbilical cord blood, a source of predominantly naîve T cells.

Tags

Cytotoxic T Lymphocytes Umbilical Cord Blood Cytomegalovirus Epstein-Barr Virus Adenovirus Stem Cell Transplantation Cord Blood Transplantation Virus-specific CTL Dendritic Cells Adenoviral Vector CMV Antigen Pp65 IL-7 IL-12 IL-15 EBV-transformed B Cells EBV-LCL
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