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Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
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Immunology and Infection
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JoVE Journal Immunology and Infection
Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus

Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus

DOI:
10.3791/3627-v

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11:18 min

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May 07, 2012

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, , ,
1Center for Cell and Gene Therapy,Baylor College of Medicine , 2Pathology and Immunology,Baylor College of Medicine , 3Department of Stem Cell Transplantation and Cellular Therapy,University of Texas M.D. Anderson Cancer Center, 4Medicine,Baylor College of Medicine , 5Department of Pediatrics,Baylor College of Medicine

Chapters

  • 00:05Title
  • 02:14Mononuclear Cell Isolation
  • 03:53Dendritic Cell (DC) Generation
  • 05:35Generation of EBV-LCL
  • 06:20CTL Initiation
  • 09:22Representative Results
  • 10:34Conclusions

Summary

Automatic Translation

Automatic Translation

Here we describe the first good manufacturing practice (GMP)-compliant method of producing virus-specific cytotoxic T lymphocytes (CTL) from umbilical cord blood, a source of predominantly naîve T cells.

Tags

Cytotoxic T LymphocytesUmbilical Cord BloodCytomegalovirusEpstein-Barr VirusAdenovirusStem Cell TransplantationCord Blood TransplantationVirus-specific CTLDendritic CellsAdenoviral VectorCMV Antigen Pp65IL-7IL-12IL-15EBV-transformed B CellsEBV-LCL

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