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JoVE Journal
Bioengineering

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In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
 

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage

Article DOI: 10.3791/3628
January 11th, 2012

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Summary January 11th, 2012

We demonstrate the assembly and application of a molecular-scale device powered by a topoisomerase protein. The construct is a bio-molecular sensor which labels two major types of DNA breaks in tissue sections by attaching two different fluorophores to their ends.

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