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Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
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Chapters
- 00:05Title
- 01:29Maize Kernal Bioassay
- 03:46Conidia Enumeration and Aflatoxin Quantification
- 05:46Fumonisin B1 Analysis
- 07:13Ergosterol Analysis
- 08:01Representative Fungal Growth Results
- 09:16Conclusion
The devastation of cereal crops by seed-infecting fungi has prompted numerous research efforts to better understand plant-pathogen interactions. To study seed-fungal interactions in a laboratory setting, we developed a robust method for the quantification of fungal reproduction, biomass, and mycotoxin contamination using kernel bioassays.
Tags
QuantificationFungal ColonizationSporogenesisMycotoxinsKernel BioassaysSeed-infecting FungiCereal ProductionMaizePathogen-induced LossesGrain IntegrityMycotoxin Seed ContaminationAflatoxinFumonisinAspergillus FlavusFusarium VerticillioidesMolecular Plant-pathogen InteractionsStandardized MethodQuantifying Biological ParametersCross-interpretationKernel AssaysQuantitative AnalysesFungal GrowthBiomassMycotoxin Contamination
