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Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging
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Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging

English

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32,176 Views

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10:16 min

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February 10, 2012

DOI:

10.3791/3740-v

DOI:
10.3791/3740-v

•

10:16 min
February 10, 2012

•

7 Views
, , , ,
1Department of Pathology, Center for Cardiovascular Biology, and Institute for Stem Cell and Regenerative Medicine,University of Washington, 2Departments of Bioengineering and Medicine/Cardiology,University of Washington

Summary

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Visualization of the coronary vessels is critical to advancing our understanding of cardiovascular diseases. Here we describe a method for perfusing murine coronary vasculature with a radiopaque silicone rubber (Microfil), in preparation for micro-Computed Tomography (μCT) imaging.

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Weyers, J. J., Carlson, D. D., Murry, C. E., Schwartz, S. M., Mahoney, Jr., W. M. Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging. J. Vis. Exp. (60), e3740, doi:10.3791/3740 (2012).

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