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Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging
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Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging

Article DOI: 10.3791/3740 • 10:16 min • February 10th, 2012
February 10th, 2012 •
1, 1, 1,2, 1, 1
1Department of Pathology, Center for Cardiovascular Biology, and Institute for Stem Cell and Regenerative Medicine, University of Washington, 2Departments of Bioengineering and Medicine/Cardiology, University of Washington

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Summary
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Visualization of the coronary vessels is critical to advancing our understanding of cardiovascular diseases. Here we describe a method for perfusing murine coronary vasculature with a radiopaque silicone rubber (Microfil), in preparation for micro-Computed Tomography (μCT) imaging.

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Retrograde Perfusion Filling Mouse Coronary Vasculature Micro Computed Tomography Imaging Visualization Imaging Techniques Vascular Network Resolution Vascular Casting Techniques Micro-Computed Tomography (µCT) Radiopaque Compound Accurate Representation Filling Technique In Vivo Cannulation Retrograde Perfusion Langendorff Perfusion System
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